Non-Human Primate CCL19/MIP-3b Cartridge

Name: Non-Human Primate CCL19/MIP-3b Cartridge
Brand: ProteinSimple
SKU: SPCKB-NP-011294

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-011294

Simple Plex Non-Human Primate MIP-3b assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.35 pg/mL
Assay Range:	3.93 - 6,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Non-Human Primate CCL19/MIP-3b Cartridge Summary:	Simple Plex assay for the detection of non-human primate Macrophage Inflammatory Protein 3 beta (MIP-3 beta ) in serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Non-Human Primate CCL19/MIP-3b Cartridge

Technical Data
Citations

Product Specifications for Non-Human Primate CCL19/MIP-3b Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Non-human Primate
Specificity	Natural and recombinant MIP-3 beta.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	37	36
Mean (pg/mL)	36.4	2055	34.8	1881
Standard Deviation	1.57	115	3.51	209
CV%	4.3	5.6	10.1	11.1

Recovery

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	86	79-101
Heparin Plasma (n=5)	82	78-88
Serum (n=5)	81	76-89

Linearity

Samples containing and/or spiked with high concentrations of MIP-3 beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Non-Human Primate CCL19/MIP-3b Cartridge

	Non-Human Primate CCL19/MIP-3b Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL19/MIP-3 beta

Macrophage inflammatory protein 3 beta (MIP-3 beta), also known as ELC (EBI1-ligand chemokine) and exodus-3, is constitutively expressed in various lymphoid tissues, including thymus, lymph nodes, appendix and spleen. It is distantly related to other CC chemokines (20-30% amino acid sequence identity).

Entrez Gene IDs

6363 (Human); 24047 (Mouse); 362506 (Rat)

Alternate Names

Beta-chemokine exodus-3, C-C motif chemokine 19, CC chemokine ligand 19, CCL19, CKb11, EBI1-ligand chemokine, ELC, ELCMIP-3-beta, Epstein-Barr virus-induced molecule 1 ligand chemokine, MGC34433, MIP-3 beta, MIP-3b, MIP3 beta, MIP3BCK beta-11, Macrophage inflammatory protein 3 beta, SCYA19EBI1 ligand chemokine, Small-inducible cytokine A19, beta chemokine exodus-3, chemokine (C-C motif) ligand 19, exodus-3, macrophage inflammatory protein 3-beta, small inducible cytokine subfamily A (Cys-Cys), member 19

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Non-Human Primate CCL19/MIP-3b Cartridge

Product Datasheets

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COA

Protocols & Manuals

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