Non-Human Primate Fibronectin Cartridge

Name: Non-Human Primate Fibronectin Cartridge
Brand: ProteinSimple
SKU: SPCKB-NP-011354

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-011354

Simple Plex Non-Human Primate Fibronectin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	0.027 ng/mL
Assay Range:	0.08 - 796 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Non-Human Primate Fibronectin Cartridge Summary:	Simple Plex assay for the detection of non-human primate Fibronectin in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Non-Human Primate Fibronectin Cartridge

Technical Data
Citations

Product Specifications for Non-Human Primate Fibronectin Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Non-human Primate
Specificity	Natural and recombinant Fibronectin.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	56	56
Mean (ng/mL)	6.6	297	5.98	283
Standard Deviation	0.159	9.9	0.505	17.5
CV%	2.4	3.3	8.5	6.2

Linearity

Samples containing and/or spiked with high concentrations of Fibronectin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Non-Human Primate Fibronectin Cartridge

	Non-Human Primate Fibronectin Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fibronectin

Fibronectin is an extracellular matrix (ECM) component and is one of the primary cell adhesion molecules. The occurrence of different isoforms is due to alternative mRNA splicing of the ED-A, ED-B and III-CS regions, and subsequent post-translational modification. Although non-reactive with adhesion receptors in its soluble state, fibronectin is highly adhesive when immobilized on a surface.

Item	Value
Entrez Gene IDs	2335 (Human); 280794 (Bovine)
Alternate Names	CIG, ED-B, FINC, FN, FN1, FNZ, Fibronectin, GFND, GFND2, LETS, MSF, SMDCF, fibronectin 1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Non-Human Primate Fibronectin Cartridge

Product Datasheets

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Protocols & Manuals

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