Non-Human Primate IL-6 Cartridge

Name: Non-Human Primate IL-6 Cartridge
Brand: ProteinSimple
SKU: SPCKB-NP-011462

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-011462

Simple Plex Non-Human Primate IL-6 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.11 pg/mL
Assay Range:	0.28 - 2,652 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Non-Human Primate IL-6 Cartridge Summary:	Simple Plex assay for the detection of non-human primate Interleukin 6 (IL-6) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Non-Human Primate IL-6 Cartridge

Technical Data
Citations

Product Specifications for Non-Human Primate IL-6 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Non-human Primate
Specificity	Natural and recombinant IL-6.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	40	40
Mean (pg/mL)	23.1	876	23.6	1008
Standard Deviation	0.899	72.1	1.82	77.7
CV%	3.9	8.2	7.7	7.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=5)	94	82-100
Heparin Plasma (n=4)	95	89-101
Serum (n=5)	95	80-101

Linearity

Samples containing and/or spiked with high concentrations of IL-6 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Non-Human Primate IL-6 Cartridge

	Non-Human Primate IL-6 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.

Item	Value
Long Name	Interleukin 6
Entrez Gene IDs	3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names	B-cell differentiation factor, B-cell stimulatory factor 2, BSF-2, BSF2, CDF, CTL differentiation factor , HSF, IFN-beta-2, IFNB2, IL-6, IL6, Interferon beta-2, MGI-2A, hybridoma growth factor, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6

Citations for Non-Human Primate IL-6 Cartridge

View all citations for the Simple Plex platform.
Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Non-Human Primate IL-6 Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

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Non-Human Primate IL-6 Cartridge

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-011462

Customize Your Simple Plex Cartridge

Key Product Details

Key Product Details

Product Specifications for Non-Human Primate IL-6 Cartridge

Precision

Recovery

Linearity

Scientific Data Examples for Non-Human Primate IL-6 Cartridge

Preparation and Storage

Background: IL-6

Citations for Non-Human Primate IL-6 Cartridge

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Product Documents for Non-Human Primate IL-6 Cartridge

Product Datasheets

COA

Certificate of Analysis

Protocols & Manuals

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