Simple Plex Cell Supernatant 1 Panel

Name: Simple Plex Cell Supernatant 1 Panel
Brand: ProteinSimple
SKU: ST01C-CS-005504

ProteinSimple, part of Bio-Techne | Catalog # ST01C-CS-005504

COA

Simple Plex 32x4 panel containing IL-12 p70, IL-15, IL-18/IL-1F4, and IL-2 with sample diluent SD13. Part # starting with ST01 ships in 2-3 days. Sample type indicated with CS (cell culture).

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Key Product Details

Features:

The Simple Plex Human Multianalyte Assay is for the detection of human IL-12 p70, IL-15, IL-18/IL-1F4, and IL-2 in cell culture supernatant samples.

Sample Type & Volume Required Per Well	Cell Culture Supernatant (25 uL)
Sensitivity	IL-12 p70 0.46 pg/mL; IL-15 0.2 pg/mL; IL-18/IL-1F4 0.96 pg/mL; IL-2 0.54 pg/mL
Assay Range	IL-12 p70 0.46 - 2,570 pg/mL; IL-15 0.2 - 1,950 pg/mL; IL-18/IL-1F4 0.96 - 3,660 pg/mL; IL-2 0.54 - 2,050 pg/mL

Summary for Simple Plex Cell Supernatant 1 Panel

Product Specifications

Reactivity	Human
Specificity	Natural and recombinant human IL-12 p70, IL-15, IL-18/IL-1F4, and IL-2.
Cross-reactivity
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

		Intra-Assay Precision		Inter-Assay Precision
	Sample	1	2	1	2
IL-12 p70	n	16	16	16	16
	Mean (pg/mL)	36.2	1657	33.2	1587
	Standard Deviation	1.25	90	1.95	137
	CV (%)	3.4	5.4	5.9	8.6
IL-15	n	16	16	16	16
	Mean (pg/mL)	10.3	540	10.8	562
	Standard Deviation	0.666	29.6	0.598	40.2
	CV (%)	6.5	5.5	5.6	7.2
IL-18/IL-1F4	n	16	16	16	16
	Mean (pg/mL)	10.5	569	10.3	587
	Standard Deviation	0.524	18.7	0.882	58.4
	CV (%)	5.2	3.3	8.6	10
IL-2	n	16	16	16	16
	Mean (pg/mL)	15	648	13	608
	Standard Deviation	0.8	32.7	1.13	34
	CV (%)	5.3	5.1	8.7	5.6

Product Specifications for Simple Plex Cell Supernatant 1 Panel

Applications

Simple Plex Automated Immunoassay

Preparation & Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Assay Procedure

IL-12 p70, IL-15, IL-18/IL-1F4, and IL-2

Simple Plex Cell Supernatant 1 Panel

ProteinSimple, part of Bio-Techne | Catalog # ST01C-CS-005504

Key Product Details

Summary for Simple Plex Cell Supernatant 1 Panel

Product Specifications

Precision

Product Specifications for Simple Plex Cell Supernatant 1 Panel

Preparation & Storage

Assay Procedure

Product Documents for Simple Plex Cell Supernatant 1 Panel

COA

Certificate of Analysis

CONTACT INFORMATION

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