Simple Plex Cell Supernatant 2 Panel

Name: Simple Plex Cell Supernatant 2 Panel
Brand: ProteinSimple
SKU: ST01C-CS-005511

ProteinSimple, part of Bio-Techne | Catalog # ST01C-CS-005511

COA

Simple Plex 32x4 panel containing Granzyme B, IFN-gamma 3rd Gen, IL-8/CXCL8, and TNF-alpha 2nd Gen with sample diluent SD13. Part # starting with ST01 ships in 2-3 days. Sample type indicated with CS (cell culture).

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Key Product Details

Features:

The Simple Plex Human Multianalyte Assay is for the detection of human GRANB, IFN gamma, IL-8, and TNF-alpha in cell culture supernatant samples.

Sample Type & Volume Required Per Well	Cell Culture Supernatant (25 uL)
Sensitivity	GRANB 1.31 pg/mL; IFN gamma 0.17 pg/mL; IL-8 0.19 pg/mL; TNF-alpha 0.3 pg/mL
Assay Range	GRANB 1.31-5,000 pg/mL; IFN gamma 0.17-4,000 pg/mL; Il-8 0.19 - 1,804 pg/mL; TNF-alpha 0.3-1,160 pg/mL

Summary for Simple Plex Cell Supernatant 2 Panel

Product Specifications

Reactivity	Human
Specificity	Natural and recombinant human GRANB, IFN gamma, IL-8 and TNF alpha.
Cross-reactivity
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

		Intra-Assay Precision		Inter-Assay Precision
	Sample	1	2	1	2
GRANB	n	16	16	16	16
	Mean (pg/mL)	28	1357	28.7	1377
	Standard Deviation	1.26	47.1	2.87	143
	CV (%)	4.5	3.5	10	10.4
IFN gamma 3rd gen	n	16	16	16	16
	Mean (pg/mL)	21.1	1175	23.4	1192
	Standard Deviation	1.48	33.4	2.52	99.4
	CV (%)	7	2.8	10.8	8.3
IL-8	n	16	16	16	16
	Mean (pg/mL)	28.7	1362	27.3	1463
	Standard Deviation	1.41	89.8	1.41	125
	CV (%)	4.9	6.6	5.2	8.6
TNF alpha 2nd Gen	n	16	16	16	16
	Mean (pg/mL)	6.99	383	0.662	36.9
	Standard Deviation	0.345	20.6	7.81	408
	CV (%)	4.9	5.4	8.5	9

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

	Sample Type	Average % Recovery	Range %
GRANB	EDTA Plasma (n=4)	103	91-117
	Heparin Plasma (n=4)	95	86-107
	Serum (n=4)	105	91-119
IFN gamma	EDTA Plasma (n=4)	89	88-90
	Heparin Plasma (n=4)	90	83-99
	Serum (n=4)	95	83-102
IL-8	EDTA Plasma (n=5)	89	75-102
	Heparin Plasma (n=5)	95	88-102
	Serum (n=3)	92	88-97
TNF alpha 2nd Gen	EDTA Plasma (n=4)	97	89-105
	Heparin Plasma (n=4)	89	77-96
	Serum (n=4)	99	92-108

Product Specifications for Simple Plex Cell Supernatant 2 Panel

Applications

Simple Plex Automated Immunoassay

Preparation & Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Assay Procedure

GRANB, IFNg 3rd gen, IL-8,TNF-a 2nd