Simple Plex Cytokine Release Panel 2

Name: Simple Plex Cytokine Release Panel 2
Brand: ProteinSimple
SKU: ST01C-PS-009470

ProteinSimple, part of Bio-Techne | Catalog # ST01C-PS-009470

COA

Simple Plex 32x4 panel containing IL-1b,IL-6 3rd gen,IL-8,TNF-a 2nd gen with sample diluent SD13. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum).

Key Product Details

Features:

The Simple Plex Human Multianalyte Assay is for the detection of human IL-1b, IL-6 3rd gen, IL-18, and TNF-a 2nd gen in plasma/serum samples.

Sample Type & Volume Required Per Well	Plasma/Serum (25 uL)
Sensitivity	IL-1b 0.064 pg/mL; IL-6 3rd gen 0.11 pg/mL; IL-8 0.08 pg/mL; TNF-a 2nd gen 0.3 pg/mL
Assay Range	IL-1b 0.4 - 1,530 pg/mL; IL-6 3rd gen 0.28 - 2,652 pg/mL; IL-8 0.19 - 1,804 pg/mL; TNF-a 2nd gen 0.3 - 1,160

Technical Data
Citations

Summary for Simple Plex Cytokine Release Panel 2

Product Specifications

Reactivity	Human
Specificity	Natural and recombinant human IL-1b, IL-6 3rd gen, IL-18, and TNF-a 2nd gen.
Cross-reactivity
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

		Intra-Assay Precision		Inter-Assay Precision
	Sample	1	2	1	2
IL-1b	n	16	16	16	16
	Mean (pg/mL)	8.66	446	8.42	410
	Standard Deviation	0.427	16.5	0.342	23.3
	CV (%)	4.9	3.7	4.0	5.7
IL-6 3rd gen	n	16	16	40	40
	Mean (pg/mL)	23.1	876	23.6	1008
	Standard Deviation	0.899	72.1	1.82	77.7
	CV (%)	3.9	8.2	7.7	7.7
IL-8	n	16	16	10	10
	Mean (pg/mL)	28.7	1362	27.3	1463
	Standard Deviation	1.41	89.8	1.41	125
	CV (%)	4.9	6.6	5.2	8.6
TNF-a 2nd gen	n	16	16	36	35
	Mean (pg/mL)	6.99	383	7.81	408
	Standard Deviation	0.345	20.6	0.662	36.9
	CV (%)	4.9	5.4	8.5	9.0

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

	Sample Type	Average % Recovery	Range %
IL-1b	EDTA Plasma (n=4)	106	97-120
	Heparin Plasma (n=4)	99	92-112
	Serum (n=4)	105	96-112
IL-6 3rd gen	EDTA Plasma (n=4)	92	86-102
	Heparin Plasma (n=4)	87	85-90
	Serum (n=4)	94	90-99
IL-8	EDTA Plasma (n=5)	89	75-102
	Heparin Plasma (n=5)	95	88-102
	Serum (n=3)	92	88-97
TNF alpha 2nd Gen	EDTA Plasma (n=4)	97	89-105
	Heparin Plasma (n=4)	89	77-96
	Serum (n=4)	99	92-108

Product Specifications for Simple Plex Cytokine Release Panel 2

Species	Human
Applications	Simple Plex Automated Immunoassay

Simple Plex Cytokine Release Panel 2

ProteinSimple, part of Bio-Techne | Catalog # ST01C-PS-009470

Key Product Details

Summary for Simple Plex Cytokine Release Panel 2

Product Specifications

Precision

Recovery

Product Specifications for Simple Plex Cytokine Release Panel 2

Preparation & Storage

Product Documents for Simple Plex Cytokine Release Panel 2

COA

Certificate of Analysis

CONTACT INFORMATION

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