Simple Plex Discovery Human CG beta Assay

Name: Simple Plex Discovery Human CG beta Assay
Brand: ProteinSimple
SKU: SPCKB-PS-012702

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-012702

Simple Plex Human CG beta assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.3 pg/mL
Assay Range:	1.31 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Discovery Human CG beta Assay Summary:	Simple Plex Discovery assay is for the detection of human Chorionic Gonadotropin Beta Chain (CG beta ) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Discovery Human CG beta Assay

Technical Data
Citations

Product Specifications for Simple Plex Discovery Human CG beta Assay

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human CG beta.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	24	25
Mean (pg/mL)	17.3	664	15	694
Standard Deviation	0.763	19.3	1.69	35.8
CV%	4.4	2.9	11.3	5.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	102	100-103
EDTA Plasma (n=2)	109	100-117
Heparin Plasma (n=2)	108	103-113
Serum (n=2)	116	115-116

Linearity

Samples containing and/or spiked with high concentrations of human CG beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Discovery Human CG beta Assay

	Simple Plex Discovery Human CG beta Ella Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Chorionic Gonadotropin beta Chain (HCG beta)

HCG is a glycoprotein hormone secreted in pregnancy by embryonic trophoblast cells during the first 7 weeks of gestation. Production subsequently shifts to the placenta, where it continues until term. HCG is a heterodimer consisting of an alpha chain and a beta chain. The alpha chain is common to several other hormones, including lutenizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH), while the beta chain is unique to HCG. Both alpha and beta chains contain a cysteine knot motif, also found in growth factors such as TGF-beta family members.

Item	Value
Long Name	Chorionic Gonadotropin beta Chain
Entrez Gene IDs	1082 (Human)
Alternate Names	CG-Beta, CGB, CGB3, CGB3choriogonadotropin subunit beta, CGB5, CGB7, CGB8, Choriogonadotropin Subunit Beta, Chorionic Gonadotropin beta Chain, Chorionic gonadotrophin chain beta, chorionic gonadotropin beta 3 subunit, chorionic gonadotropin beta subunit, chorionic gonadotropin, beta polypeptide, hCGB

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Discovery Human CG beta Assay

Product Datasheets

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Protocols & Manuals

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