Simple Plex Discovery Human DDL1 Assay

Name: Simple Plex Discovery Human DDL1 Assay
Brand: ProteinSimple
SKU: SPCKB-PS-012304

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-012304

Product Datasheet / COA

Simple Plex Human DDL1 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.69 pg/mL
Assay Range:	2.36 - 9,004 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Discovery Human DDL1 Assay Summary:	Simple Plex Discovery assay is for the detection of human Delta-like Protein 1 (DLL1) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Discovery Human DDL1 Assay

Technical Data
Citations

Product Specifications for Simple Plex Discovery Human DDL1 Assay

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human DLL1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	34	34
Mean (pg/mL)	66.6	3137	70.2	3153
Standard Deviation	2.09	85.9	7.05	269
CV%	3.1	2.7	10.1	8.5

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	120	118-121

Linearity

Samples containing and/or spiked with high concentrations of human DLL1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Discovery Human DDL1 Assay

	Simple Plex Discovery Human DLL1 Ella Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DLL1

DLL1 (Delta-like protein 1) is a transmembrane glycoprotein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. It plays a role in central nervous system morphogenesis, intervertebral joint formation, inner ear hair cell development and patterning, and arteriogenesis. It regulates the homeostasis and differentiation of neural, pancreatic, and myogenic progenitor cells, T and B lymphocytes. DLL1 is overexpressed in a number of cancers and contributes to tumorigenesis. The extracellular region of DLL1 can be shed from the cell surface, and the intracellular domain can be cleaved by gamma-secretase to translocate to the nucleus and regulate gene transcription.

Item	Value
Long Name	Delta-like 1
Entrez Gene IDs	28514 (Human); 13388 (Mouse); 84010 (Rat)
Alternate Names	DL1, DLL1, Delta, Delta 1, Delta1, Drosophila Delta homolog 1, H-Delta-1, delta (Drosophila)-like 1, delta-like 1 (Drosophila), delta-like protein 1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Discovery Human DDL1 Assay

Product Datasheets

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COA

Protocols & Manuals

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