Simple Plex Discovery Human TFPI Assay

Name: Simple Plex Discovery Human TFPI Assay
Brand: ProteinSimple
SKU: SPCKB-PS-011824

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-011824

Simple Plex Human TFPI assay kit for use on Ella instrument. Contains a cartridge, Sample Diluent SD13, and Wash Buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	0.409 pg/mL
Assay Range:	1.61 - 6,148 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Discovery Human TFPI Assay Summary:	Simple Plex Discovery assay is for the detection of human Tissue Factor Pathway Inhibitor (TFPI) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Discovery Human TFPI Assay

Technical Data
Citations

Product Specifications for Simple Plex Discovery Human TFPI Assay

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human TFPI.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	21	21
Mean (pg/mL)	48.3	2570	48.4	2396
Standard Deviation	3.751	172	5.6	140
CV%	4.9	6.7	11.6	5.9

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	108	105-111

Linearity

Samples containing and/or spiked with high concentrations of recombinant human TFPI were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Discovery Human TFPI Assay

	Simple Plex Discovery TFPI Ella Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-3

Interleukin-3 (IL-3) is a secreted cytokine that stimulates the proliferation and differentiation of pluripotent hematopoietic stem cells and various lineage-committed progenitors. It also regulates the activation of mature mast cells, basophils, eosinophils, and macrophages. It is produced by activated T cells, thymic epithelial cells, activated mast cells, keratinocytes, neurons, and astrocytes. IL-3 signals through a receptor complex that contains IL-3 R alpha/CD123 and the common beta chain which is also a component of the receptors for IL-5 and GM-CSF. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils, and various myeloid leukemic cells. Human IL-3 does not show activity on mouse cells.

Item	Value
Long Name	Interleukin 3
Entrez Gene IDs	3562 (Human); 16187 (Mouse); 24495 (Rat)
Alternate Names	Hematopoietic growth factor, IL-3, IL-3MGC79398, IL3, MCGF, MCGFMGC79399, MULTI-CSF, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell stimulating factor, P-cell-stimulating factor, interleukin 3 (colony-stimulating factor, multiple), interleukin-3, mast-cell growth factor, multilineage-colony-stimulating factor

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Discovery Human TFPI Assay

Product Datasheets

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Protocols & Manuals

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