Simple Plex Discovery Mouse Total MMP-9 Assay

Name: Simple Plex Discovery Mouse Total MMP-9 Assay
Brand: ProteinSimple
SKU: SPCKB-MP-012215

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-012215

Simple Plex Mouset Total MMP-9 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD09, and Wash Buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Mouse Cell Culture Supernates (10 uL), Mouse Serum (10 uL), Rat Serum (10 uL), Mouse EDTA Plasma (25 uL), Rat EDTA Plasma (10 uL), Mouse Heparin Plasma (25 uL), Rat Heparin Plasma (10 uL)
Sensitivity:	8.99 pg/mL
Assay Range:	27.2 - 41,458 pg/mL (Mouse Cell Culture Supernates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Mouse Heparin Plasma, Rat Heparin Plasma)
Simple Plex Discovery Mouse Total MMP-9 Assay Summary:	Simple Plex Discovery assay is for the detection of mouse and rat natural, active, and pro-Matrix Metalloproteinase 9 (total MMP-9) in cell culture supernatant (CCS), serum, and platelet-poor plasma (EDTA/Heparin) in Sample Diluent SD09. CCS is validated for mouse only.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Discovery Mouse Total MMP-9 Assay

Technical Data
Citations

Product Specifications for Simple Plex Discovery Mouse Total MMP-9 Assay

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Specificity	Recombinant MMP-9 and natural, active, pro, and TIMP-1 complexed MMP-9.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Mouse Cell Culture Supernates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Mouse Heparin Plasma, Rat Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	38	38
Mean (pg/mL)	323	14773	305	14016
Standard Deviation	10.3	577	26.1	1438
CV%	3.2	3.9	8.6	10.3

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	101	98-105

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat Total MMP-9 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Discovery Mouse Total MMP-9 Assay

	Simple Plex Discovery Mouse Total MMP-9 Ella Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-9

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Item	Value
Long Name	Matrix Metalloproteinase 9
Entrez Gene IDs	4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)
Alternate Names	92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, EC 3.4.24, EC 3.4.24.35, GELB, Gelatinase B, MANDP2, MMP-9, MMP9, macrophage gelatinase, matrix metallopeptidase 9, matrix metalloproteinase 9, matrix metalloproteinase-9, type V collagenase

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Discovery Mouse Total MMP-9 Assay

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Protocols & Manuals

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