Simple Plex Discovery Rat MMP-9 Assay

Name: Simple Plex Discovery Rat MMP-9 Assay
Brand: ProteinSimple
SKU: SPCKB-RP-014262

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-RP-014262

Product Datasheet / COA

Simple Plex Rat MMP-9 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL)
Sensitivity:	8.99 pg/mL
Assay Range:	27.2 - 41,458 pg/mL (Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
Simple Plex Discovery Rat MMP-9 Assay Summary:	Simple Plex Discovery assay is for the detection of rat natural, active, and pro-Matrix Metalloproteinase 9 (total MMP-9) in serum, and platelet-poor plasma (EDTA/Heparin) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Discovery Rat MMP-9 Assay

Technical Data
Citations

Product Specifications for Simple Plex Discovery Rat MMP-9 Assay

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Rat
Specificity	Recombinant MMP-9 and natural, active, pro, and TIMP-1 complexed MMP-9.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	38	38
Mean (pg/mL)	323	14773	305	14016
Standard Deviation	10.3	577	26.1	1438
CV%	3.2	3.9	8.6	10.3

Linearity

Samples containing and/or spiked with high concentrations of rat Total MMP-9 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Discovery Rat MMP-9 Assay

	Simple Plex Discovery Rat MMP-9 Uteroglobin Ella Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lipocalin-2/NGAL

The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.

Item	Value
Long Name	Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs	3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names	24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL, LCN2, Lipocalin-2, Lipocalin2, MSFI, NGAL, NGALlipocalin-2, Oncogene 24p3, Siderocalin, Uterocalin, lipocalin 2, lipocalin 2 (oncogene 24p3), migration-stimulating factor inhibitor, neutrophil gelatinase-associated lipocalin, p25

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Discovery Rat MMP-9 Assay

Product Datasheets

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COA

Protocols & Manuals

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