Simple Plex HIV-1 Gag p24 Cartridge

Name: Simple Plex HIV-1 Gag p24 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000981

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000981

Product Datasheet / COA

This product has been discontinued and replaced with the HIVp24 2nd generation Simple Plex Assay.

Discontinued Product

SPCKB-PS-000981 has been discontinued. View all HIV-1 Gag p24 products.

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.67 pg/mL
Assay Range:	3.93 - 15,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex HIV-1 Gag p24 Cartridge Summary:	Simple Plex assay for the detection of HIV Gag p24 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) on Ella automated benchtop ELISA.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex HIV-1 Gag p24 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex HIV-1 Gag p24 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Specificity	Natural and recombinant HIV-1 Gag p24.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	35	34
Mean (pg/mL)	105	4500	94.3	4074
Standard Deviation	0.37	237	9.64	377
CV%	7.1	5.3	10.2	9.3

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	114	111-118
EDTA Plasma (n=4)	107	102-116
Heparin Plasma (n=4)	107	99-112
Serum (n=4)	111	109-113

Linearity

Samples containing and/or spiked with high concentrations of human HIV Gag p24 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex HIV-1 Gag p24 Cartridge

	HIV Gag p24 Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55^Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Item	Value
Long Name	Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa
Entrez Gene IDs	155030 (Viral)
Alternate Names	CA, Capsid Protein, HIV-1 Gag p24, HIV1 Gag p24, Pr55(Gag)

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex HIV-1 Gag p24 Cartridge

Product Datasheets

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COA

Protocols & Manuals

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