Simple Plex HIV-1 p24 2nd Gen Cartridge

Name: Simple Plex HIV-1 p24 2nd Gen Cartridge
Brand: ProteinSimple
SKU: SPCKB-CS-009216

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-CS-009216

Simple Plex HIV-1 p24 2nd Gen assay kit for use on Ella instrument. Contains cartridge, sample diluent SD30, and wash buffer.

Need a different panel or sample type? Customize your cartridge here.

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL)
Sensitivity:	5.1 pg/mL
Assay Range:	11.7 - 112,000 pg/mL (Cell Culture Supernates)
Simple Plex HIV-1 p24 2nd Gen Cartridge Summary:	Simple Plex assay for the detection of HIV-1 p24 in cell culture supernatant (CCS).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex HIV-1 p24 2nd Gen Cartridge

Technical Data
Citations

Product Specifications for Simple Plex HIV-1 p24 2nd Gen Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Specificity	This assay recognizes HIV-1 p24.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	29	29
Mean (µL)	782	36099	856	37492
Standard Deviation	38	2120	93.1	2511
CV%	4.9	5.9	10.9	6.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media Pretreated (n=7)	101	91-111
Cell Culture Media Untreated (n=7)	104	90-115

Linearity

Samples containing and/or spiked with high concentrations of HIV-1 p24 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex HIV-1 p24 2nd Gen Cartridge

	HIV-1 p24 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55^Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Item	Value
Long Name	Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa
Entrez Gene IDs	155030 (Viral)
Alternate Names	CA, Capsid Protein, HIV-1 Gag p24, HIV1 Gag p24, Pr55(Gag)

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex HIV-1 p24 2nd Gen Cartridge

Product Datasheets

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Protocols & Manuals

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