Simple Plex Human Amphiregulin Cartridge

Name: Simple Plex Human Amphiregulin Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-001181

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001181

Simple Plex Human Amphiregulin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

Customize Your Simple Plex Cartridge

Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.

Build My Cartridge

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	2.62 pg/mL
Assay Range:	5.41 - 51,610 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Amphiregulin Cartridge Summary:	Simple Plex assay for the detection of human Amphiregulin in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Amphiregulin Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Amphiregulin Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Amphiregulin.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	69	69
Mean (pg/mL)	73.7	3225	68.1	3228
Standard Deviation	3.19	236	7.01	285
CV%	4.3	7.3	10.3	8.8

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	83	81-85
Heparin Plasma (n=4)	83	79-86
Serum (n=4)	84	83-85

Linearity

Samples containing and/or spiked with high concentrations of human Amphiregulin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Amphiregulin Cartridge

	Human Amphiregulin Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Amphiregulin

Amphiregulin (AR) is an EGF family growth factor that is released as a soluble protein following proteolytic cleavage of its transmembrane precursor. Amphiregulin is expressed by numerous carcinoma cell lines and epithelial cells of the colon, stomach, breast, ovary and kidney. It acts through ErbB family of receptor to stimulate the proliferation of keratinocytes, mammary epithelial cells, fibroblasts, astrocytes and glial cells. Amphiregulin can also inhibit the growth of certain tumor cells.

Item	Value
Entrez Gene IDs	374 (Human); 11839 (Mouse)
Alternate Names	AR, AREG, AREGB, Amphiregulin, CRDGF, Colorectum cell-derived growth factor, MGC13647, SDGF, schwannoma-derived growth factor

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

Learn More

Product Documents for Simple Plex Human Amphiregulin Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol