Simple Plex Human Axl Cartridge

Name: Simple Plex Human Axl Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000472

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000472

Product Datasheet / COA

Simple Plex Human Axl assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity:	10.4 pg/mL
Assay Range:	48.72 - 74,340 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Axl Cartridge Summary:	Simple Plex assay for the detection of human Axl in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Axl Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Axl Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Axl.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	23	25
Mean (pg/mL)	420	19305	383	19730
Standard Deviation	16.3	1708	26.4	1794
CV%	3.9	8.8	6.9	9.1

Linearity

Samples containing and/or spiked with high concentrations of human Axl were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Axl Cartridge

	Human Axl Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse orthologs of chicken c-Eyk) constitute the TAM receptor tyrosine kinase subfamily. This RTK subfamily is characterized by an extracellular domain that consists of two Ig-like motifs and two fibronectin type III motifs. These receptors bind the vitamin K-dependent protein Growth Arrest Specific Gene 6 (Gas6). Receptor activation leads to cell proliferation, migration, or the prevention of apoptosis. Cellular signaling through this family of RTKs is involved in hematopoiesis, embryonic development, tumorigenesis, and spermatogenesis.

Item	Value
Long Name	Axl Receptor Tyrosine Kinase
Entrez Gene IDs	558 (Human); 26362 (Mouse); 102126361 (Cynomolgus Monkey)
Alternate Names	AI323647, ARK, AXL oncogene, AXL receptor tyrosine kinase, AXL transforming sequence/gene, Axl, EC 2.7.10, EC 2.7.10.1, JTK11, Tyro7, UFO, UFOoncogene AXL, tyrosine-protein kinase receptor UFO

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Axl Cartridge

Product Datasheets

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COA

Protocols & Manuals

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