Simple Plex Human BAFF/BLyS/TNFSF13B Cartridge

Name: Simple Plex Human BAFF/BLyS/TNFSF13B Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000243

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000243

Product Datasheet / COA

Simple Plex Human BAFF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Cerebrospinal Fluid (25 uL)
Sensitivity:	0.212 pg/mL
Assay Range:	0.55 - 840 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Human BAFF/BLyS/TNFSF13B Cartridge Summary:	Simple Plex assay for the detection of human B Cell Activating Factor belonging to the TNF Family (BAFF) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human BAFF/BLyS/TNFSF13B Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human BAFF/BLyS/TNFSF13B Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human BAFF.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	22	22
Mean (pg/mL)	5.54	241	5.48	242
Standard Deviation	0.434	15.9	0.266	26.7
CV%	7.8	6.6	4.8	11

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	100	100-100

Linearity

Samples containing and/or spiked with high concentrations of human BAFF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human BAFF/BLyS/TNFSF13B Cartridge

	Human BAFF/BLyS Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BAFF/BLyS/TNFSF13B

BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.

Item	Value
Long Name	B cell Activating Factor
Entrez Gene IDs	10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)
Alternate Names	ApoL related ligand TALL-1, B lymphocyte stimulator, B-cell-activating factor, BAFF, BAFFB-cell activating factor, BLYSB-lymphocyte stimulator, BLyS, CD257, CD257 antigen, DTL, Dendritic cell-derived TNF-like molecule, TALL-1delta BAFF, TALL1, TALL1Delta4 BAFF, THANK, TNF and ApoL-related leukocyte expressed ligand 1, TNF homolog that activates apoptosis, TNF- and APOL-related leukocyte expressed ligand 1, TNFSF13B, TNFSF20, ZTNF4, tumor necrosis factor (ligand) superfamily, member 13b, tumor necrosis factor (ligand) superfamily, member 20, tumor necrosis factor ligand superfamily member 13B, tumor necrosis factor superfamily, member 13b, tumor necrosis factor-like protein ZTNF4

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human BAFF/BLyS/TNFSF13B Cartridge

Product Datasheets

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COA

Protocols & Manuals

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