Simple Plex Human BCMA/TNFRSF17 Cartridge

Name: Simple Plex Human BCMA/TNFRSF17 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000768

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000768

Simple Plex Human BCMA/TNFRSF17 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	0.54 pg/mL
Assay Range:	2.62 - 10,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human BCMA/TNFRSF17 Cartridge Summary:	Simple Plex assay for the detection of human B Cell Maturation Antigen/TNFRSF17 (BCMA) in serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human BCMA/TNFRSF17 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human BCMA/TNFRSF17 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human BCMA.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	35	35
Mean (pg/mL)	58.8	2931	57.7	2971
Standard Deviation	2.96	170	6.4	283
CV%	5	5.8	11.1	9.5

Linearity

Samples containing and/or spiked with high concentrations of human BCMA were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human BCMA/TNFRSF17 Cartridge

	Human BCMA Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BCMA/TNFRSF17

B cell maturation protein (BCMA) is a member of the TNF receptor superfamily (TNFRSF17). BCMA is expressed in immune organs and mature Bcell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. BCMA binding to APRIL or BAFF stimulates IgM production in peripheral blood B cells and increases the survival of cultured B cells. Mature human and mouse BCMA share 86% amino acid sequence identity.

Item	Value
Long Name	B Cell Maturation Factor
Entrez Gene IDs	608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)
Alternate Names	B cell maturation antigen, B-cell maturation protein, BCMA, BCMAtumor necrosis factor receptor superfamily member 17, BCMB-cell maturation factor, CD269, CD269 antigen, TNFRSF13A, TNFRSF17, tumor necrosis factor receptor superfamily, member 17

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human BCMA/TNFRSF17 Cartridge

Product Datasheets

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Protocols & Manuals

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