Simple Plex Human Beta-NGF Cartridge

Name: Simple Plex Human Beta-NGF Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000461

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000461

Please use SPCKB-PS-000623: Simple Plex Human beta-NGF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

Discontinued Product

SPCKB-PS-000461 has been discontinued. An alternative/replacement product is available: SPCKB-PS-000623. View all beta-NGF products.

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.29 pg/mL
Assay Range:	0.69 - 6,600 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Beta-NGF Cartridge Summary:	Simple Plex assay for the detection of human beta -NGF in serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Beta-NGF Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Beta-NGF Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human beta -NGF.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) control was Each tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	17
Mean (pg/mL)	19.3	1131	19.6	1034
Standard Deviation	1.68	101	2.22	96.5
CV%	8.7	8.9	11.3	9.3

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.<br>

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	89	74-110
Heparin Plasma (n=4)	96	88-109
Serum (n=4)	90	83-95

Linearity

Samples containing and/or spiked with high concentrations of human beta -NGF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Beta-NGF Cartridge

	Human beta -NGF Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: beta-NGF

Nerve growth factor (NGF) was initially isolated in the mouse submandibular gland over three decades ago as a 7S complex composed of three non- covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases, while the beta subunit, called beta-NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. The human protein shares approximately 90% identity at the amino acid level with both mouse and rat beta-NGF.

Item	Value
Long Name	beta Nerve Growth Factor
Entrez Gene IDs	4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)
Alternate Names	Beta nerve growth factor precursor, HSAN5, MGC161426, NGFB, NGFBMGC161428, beta-NGF, beta-nerve growth factor, betaNGF, nerve growth factor (beta polypeptide), nerve growth factor, beta subunit

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Beta-NGF Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol