Are the chelating properties of EDTA plasma samples an issue for the Simple Plex Human CD163 Assay?

Name: Simple Plex Human CD163 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000479

The Simple Plex Human CD163 assay works well with EDTA plasma samples. In house validation of EDTA plasma samples meets all specifications.

Simple Plex Human CD163 Cartridge

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000479

Product Datasheet / COA

DISCONTINUED - please use CD163 2nd gen. SPCKB-PS-007509.

Discontinued Product

SPCKB-PS-000479 has been discontinued. View all CD163 products.

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (500 nL), EDTA Plasma (500 nL), Heparin Plasma (500 nL)
Sensitivity:	1986 pg/mL
Assay Range:	1986 - 484,850 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human CD163 Cartridge Summary:	Simple Plex assay for the detection of human CD163 in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CD163 Cartridge

Technical Data
Citations / FAQs

Product Specifications for Simple Plex Human CD163 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human CD163.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	16
Mean (pg/mL)	3275	160972	3030	153722
Standard Deviation	262	14297	335	9371
CV%	8	8.9	11	6.1

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	96	86-108
Heparin Plasma (n=4)	93	83-97
Serum (n=4)	98	91-109

Linearity

Samples containing and/or spiked with high concentrations of human CD163 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human CD163 Cartridge

	Human CD163 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD163

CD163, also known as M130 and p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.

Item	Value
Entrez Gene IDs	9332 (Human); 93671 (Mouse); 312701 (Rat)
Alternate Names	CD163, CD163 molecule, CD_antigen: CD163, GHI/61, HbSR, Hemoglobin scavenger receptor, M130, MM130, RM3/1, SCARI1, Soluble CD163, macrophage-associated antigen, sCD163, scavenger receptor cysteine-rich type 1 protein M130

FAQs for Simple Plex Human CD163 Cartridge

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CD163 Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol