Simple Plex Human CTLA-4 Cartridge

Name: Simple Plex Human CTLA-4 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-003114

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-003114

Simple Plex Human CTLA-4 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.1 pg/mL
Assay Range:	3.14 - 30,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human CTLA-4 Cartridge Summary:	Simple Plex assay for the detection of human CTLA-4 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CTLA-4 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CTLA-4 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human CTLA-4.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	54	51
Mean (pg/mL)	209	9821	212	9517
Standard Deviation	8.97	616	16.4	701
CV%	4.3	6.3	7.7	7.4

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	89	83-99
Heparin Plasma (n=4)	87	84-90
Serum (n=4)	95	89-100

Linearity

Samples containing and/or spiked with high concentrations of human CTLA-4 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human CTLA-4 Cartridge

	Human CTLA-4 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CTLA-4

CTLA-4/CD152 (Cytotoxic T-Lymphocyte Antigen 4) is a transmembrane T cell inhibitory molecule that is expressed as a covalent homodimer. CTLA-4 is recruited from intracellular vesicles to the immunological synapse beginning 1-2 days after T cell activation. It forms a linear lattice with B7-1 on APC, inducing negative regulatory signals and ending CD28-dependent T cell activation. Mice deleted for CTLA-4 develop lethal autoimmune reactions due to continued T cell activation and poor control by regulatory T cells which constitutively express CTLA-4.

Item	Value
Long Name	Cytotoxic T-lymphocyte-associated Molecule 4
Entrez Gene IDs	1493 (Human); 12477 (Mouse); 403696 (Canine); 102115124 (Cynomolgus Monkey)
Alternate Names	CD, CD152, CD152 antigen, CD152IDDM12, CD28, CELIAC3, CTLA-4, CTLA4, Cytotoxic T-lymphocyte-associated antigen 4, GRD4, GSE, ICOS, celiac disease 3, cytotoxic T-lymphocyte antigen 4, cytotoxic T-lymphocyte protein 4, cytotoxic T-lymphocyte-associated protein 4, cytotoxic T-lymphocyte-associated serine esterase-4, ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CTLA-4 Cartridge

Product Datasheets

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Protocols & Manuals

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