Simple Plex Human CXCL11/I-TAC Cartridge

Name: Simple Plex Human CXCL11/I-TAC Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000369

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000369

Simple Plex Human CXCL11/I-TAC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD16, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	0.181 pg/mL
Assay Range:	0.545 - 2,080 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human CXCL11/I-TAC Cartridge Summary:	Simple Plex assay for the detection of human I-TAC in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CXCL11/I-TAC Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CXCL11/I-TAC Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human I-TAC.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	8	5
Mean (pg/mL)	22	1212	21.9	1164
Standard Deviation	0.809	83.9	1.31	173
CV%	3.7	6.9	6	14.9

Linearity

Samples containing and/or spiked with high concentrations of human I-TAC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human CXCL11/I-TAC Cartridge

	Human I-TAC Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL11/I-TAC

Interferon-inducible T cell a chemoattractant (I-TAC), also known as SCYB9B, H174 and beta-R1, is a non-ELR motif-containing CXC chemokine. I-TAC shares 36% and 37% amino acid sequence homology with IP-10 and MIG, respectively. I-TAC is expressed at low levels in normal tissues, including thymus, spleen and pancreas.

Item	Value
Entrez Gene IDs	6373 (Human); 56066 (Mouse)
Alternate Names	CXCL11, H174, H174IP9, I-TAC, I-TACMGC102770, IP-9member 11, ITAC, Interferon gamma-inducible protein 9, Interferon-inducible T-cell alpha chemoattractant, SCYB9B, Small-inducible cytokine B11, b-R1, beta-R1, chemokine (C-X-C motif) ligand 11, small inducible cytokine subfamily B (Cys-X-Cys), member 9B

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CXCL11/I-TAC Cartridge

Product Datasheets

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COA

Protocols & Manuals

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