Simple Plex Human Cytokeratin 18 Cartridge

Name: Simple Plex Human Cytokeratin 18 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-001065

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001065

Simple Plex Human Cytokeratin 18 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	8.52 pg/mL
Assay Range:	141 - 86,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Cytokeratin 18 Cartridge Summary:	Simple Plex assay for the detection of human Cytokeratin 18 in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Cytokeratin 18 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Cytokeratin 18 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Cytokeratin 18.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	38	40
Mean (pg/mL)	522	33577	473	29354
Standard Deviation	48.9	3085	50	2606
CV%	9.4	9.2	10.6	8.9

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	104	86-116
Heparin Plasma (n=4)	100	81-111
Serum (n=4)	105	80-115

Linearity

Samples containing and/or spiked with high concentrations of human Cytokeratin 18 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Cytokeratin 18 Cartridge

	Human Cytokeratin 18 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Cytokeratin 18

Cytokeratins are cytoskeletal intermediate filament proteins that are classified as type I (acidic) and type II (neutral to basic) and associate into heterotetramers.

Item	Value
Entrez Gene IDs	3875 (Human); 16668 (Mouse)
Alternate Names	CK-18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin 18, K18, KRT18, Keratin 18, cell proliferation-inducing protein 46, cytokeratin-18, keratin, type I cytoskeletal 18, keratin-18

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Cytokeratin 18 Cartridge

Product Datasheets

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Protocols & Manuals

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