Simple Plex Human Endoglin/CD105 Cartridge

Name: Simple Plex Human Endoglin/CD105 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000371

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000371

Simple Plex Human Endoglin/CD105 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity:	5.66 pg/mL
Assay Range:	15.4 - 147,150 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Endoglin/CD105 Cartridge Summary:	Simple Plex assay for the detection of human Endoglin in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Endoglin/CD105 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Endoglin/CD105 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Endoglin.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	17
Mean (pg/mL)	951	45292	1001	49876
Standard Deviation	90.5	1760	74.9	4456
CV%	9.5	3.9	7.5	8.9

Linearity

Samples containing and/or spiked with high concentrations of human Endoglin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Endoglin/CD105 Cartridge

	Human Endoglin Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Endoglin/CD105

Endoglin (CD105) is a transmembrane type III receptor for TGF-beta superfamily ligands and plays an important role in smooth muscle differentiation, angiogenesis, and neovascularization. It is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. Human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I. Elevated levels of anti-angiogenic soluble Endoglin contribute to pathogenicity in preeclampsia. Endoglin associates with the receptors TGF-beta RII, Activin RIIA or RIIB, BMPR-IA/ALK-3, or BMPR-IB/ALK6 and enhance binding of Activin A, BMP-2, -7, -9, TGF-beta 1, or TGF-beta 3. Endoglin can either enhance or inhibit signaling through these receptor complexes.

Item	Value
Entrez Gene IDs	2022 (Human); 13805 (Mouse); 497010 (Rat)
Alternate Names	CD105, CD105 antigen, ENDOsler-Rendu-Weber syndrome 1, ENG, Endoglin, HHT1FLJ41744, ORW, ORW1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Endoglin/CD105 Cartridge

Product Datasheets

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COA

Protocols & Manuals

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