Simple Plex Human ErbB2/Her2 Cartridge

Name: Simple Plex Human ErbB2/Her2 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000335

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000335

Simple Plex Human ErbB2/Her2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (25 uL)
Sensitivity:	2.54 pg/mL
Assay Range:	13.1 - 8,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Simple Plex Human ErbB2/Her2 Cartridge Summary:	Simple Plex assay for the detection of human ErbB2 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human ErbB2/Her2 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human ErbB2/Her2 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human ErbB2.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	15	35
Mean (pg/mL)	48	2043	52.3	2223
Standard Deviation	2.88	141	3.14	172
CV%	6	6.9	6	7.8

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	108	106-111
Urine (n=4)	112	97-125

Linearity

Samples containing and/or spiked with high concentrations of human ErbB2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human ErbB2/Her2 Cartridge

	Human ErbB2/Her2 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ErbB2/Her2

ErbB2, also called Neu and Her2, is a transmembrane glycoprotein in the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and over-expressed in a large number of breast carcinomas. ErbB2 has no identified ligands but heterodimerizes with ErbB1/EGF R, ErbB3, or ErbB4 to form higher affinity signaling complexes. The protease ADAM10 releases a 110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2. ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system.

Item	Value
Long Name	Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs	2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)
Alternate Names	CD340, CD340 antigen, EC 2.7.10, EGFR2, ErbB2, HER-2, HER2, HER2EC 2.7.10.1, MLN 19, MLN19, Metastatic lymph node gene 19 protein, NEUHER-2/neu, NGL, NGLTKR1, Neu Oncogene, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, TKR1, Tyrosine kinase-type cell surface receptor HER2, c-erb B2/neu protein, herstatin, neuroblastoma/glioblastoma derived oncogene homolog, p185erbB2, receptor tyrosine-protein kinase erbB-2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog), v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human ErbB2/Her2 Cartridge

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Protocols & Manuals

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