Simple Plex Human FAP Cartridge

Name: Simple Plex Human FAP Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000769

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000769

Simple Plex Human Fibroblast Activation Protein alpha/FAP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (500 nL), EDTA Plasma (500 nL), Heparin Plasma (500 nL)
Sensitivity:	0.9 pg/mL
Assay Range:	10.5 - 40,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human FAP Cartridge Summary:	Simple Plex assay for the detection of human FAP in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human FAP Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human FAP Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human FAP.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	17	17
Mean (pg/mL)	227	10581	227	11364
Standard Deviation	5.27	731	19.9	621
CV%	2.3	6.9	8.8	5.5

Linearity

Samples containing and/or spiked with high concentrations of human FAP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human FAP Cartridge

	Human FAP Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fibroblast Activation Protein alpha/FAP

FAP (fibroblast activation protein-alpha; also known as seprase) is a type II transmembrane serine protease that degrades gelatin, Type I collagen, and N-terminal prolines. FAP is induced on reactive stromal fibroblasts in epithelial cancers, sarcomas, and granulation tissue, and may play roles in tumor invasion, tissue remodeling, and wound repair.

Item	Value
Entrez Gene IDs	2191 (Human); 14089 (Mouse); 102134935 (Cynomolgus Monkey)
Alternate Names	170 kDa melanoma membrane-bound gelatinase, DKFZp686G13158, DPPIV, EC 3.4.21.-, FAP, FAPA, Fibroblast Activation Protein alpha, Integral membrane serine protease, Seprase, fibroblast activation protein, alpha, vibronectin

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human FAP Cartridge

Product Datasheets

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Protocols & Manuals

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