Simple Plex Human Fas/TNFRSF6/CD95 Cartridge

Name: Simple Plex Human Fas/TNFRSF6/CD95 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-001042

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001042

Simple Plex Human Fas/TNFRSF6/CD95 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	0.83 pg/mL
Assay Range:	5.45 - 20,792 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Fas/TNFRSF6/CD95 Cartridge Summary:	Simple Plex assay for the detection of human Fas in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Fas/TNFRSF6/CD95 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Fas/TNFRSF6/CD95 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Fas.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	16
Mean (pg/mL)	333	16033	330	17111
Standard Deviation	13	1372	22.4	1943
CV%	3.9	8.6	6.8	11.4

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=5)	101	99-104

Linearity

Samples containing and/or spiked with high concentrations of human Fas were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Fas/TNFRSF6/CD95 Cartridge

	Human Fas Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fas/TNFRSF6/CD95

Fas (Fibroblast-associated), also known as Apo-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein that binds to monoclonal antibodies that are cytolytic for various human cell lines. Alternatively spliced cDNAs encoding multiple Fas isoforms, including a soluble form of Fas, have been identified. Fas is highly expressed in epithelial cells, hepatocyes, activated mature lymphocytes, virus-transformed lymphocytes and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney and ovary.

Item	Value
Long Name	Fibroblast-associated
Entrez Gene IDs	355 (Human); 14102 (Mouse); 246097 (Rat); 102140989 (Cynomolgus Monkey); 493881 (Feline)
Alternate Names	APT1, APT1FASTM, Apo-1, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, CD95, CD95 antigen, CD95ALPS1A, FAS1, FASLG receptor, Fas, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, TNFRSF6, TNFRSF6member 6, apoptosis antigen 1, tumor necrosis factor receptor superfamily member 6

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Fas/TNFRSF6/CD95 Cartridge

Product Datasheets

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Protocols & Manuals

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