Simple Plex Human Gas6 Cartridge

Name: Simple Plex Human Gas6 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000491

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000491

Product Datasheet / COA

Simple Plex Human Gas6 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity:	1.06 pg/mL
Assay Range:	2.62 - 10,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Gas6 Cartridge Summary:	Simple Plex assay for the detection of human Gas6 in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Gas6 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Gas6 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Gas6.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	21	20
Mean (pg/mL)	55.6	2933	53.6	2787
Standard Deviation	3.31	201	3.18	184
CV%	5.9	6.9	5.9	6.6

Linearity

Samples containing and/or spiked with high concentrations of human Gas6 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Gas6 Cartridge

	Human Gas6 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Gas6

Gas6 (Growth Arrest Specific 6) is a secreted protein that contains an extensively gamma-carboxylated N-terminal Gla domain and is dependent upon vitamin K for activity. Gas6 signals through the receptors Axl, Dtk/Tyro3, and Mer and is inhibited by shed soluble forms of Axl and Mer. Gas6 protects cells from stress-induced apoptosis and promotes phagocytosis of apoptotic cell debris, promotes platelet activation but inhibits inflammatory cytokine production, and inhibits VEGF-induced angiogenesis, and regulates tumor cell invasiveness.

Item	Value
Long Name	Growth-arrest-specific Protein 6
Entrez Gene IDs	2621 (Human); 14456 (Mouse)
Alternate Names	AXLLG, AXLLGAXL stimulatory factor, AXSFAXL receptor tyrosine kinase ligand, DKFZp666G247, FLJ34709, GAS-6, Gas6, growth arrest-specific 6, growth arrest-specific protein 6

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Gas6 Cartridge

Product Datasheets

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COA

Protocols & Manuals

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