Simple Plex Human HGFR/c-MET Cartridge

Name: Simple Plex Human HGFR/c-MET Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000325

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000325

Simple Plex Human HGFR/c-MET assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (500 nL), EDTA Plasma (500 nL), Heparin Plasma (500 nL)
Sensitivity:	1.9 pg/mL
Assay Range:	4.2 - 100,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human HGFR/c-MET Cartridge Summary:	Simple Plex assay for the detection of human HGF R in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human HGFR/c-MET Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human HGFR/c-MET Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human HGF R.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	32	33
Mean (pg/mL)	299	13605	272	13483
Standard Deviation	21.2	849	23.7	1263
CV%	7.2	6.2	8.7	9.4

Linearity

Samples containing and/or spiked with high concentrations of human HGF R were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human HGFR/c-MET Cartridge

	Human HGF R Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HGFR/c-MET

HGF receptor, a product of the proto-oncogene c-met, is a heterodimeric transmembrane glycoprotein that is a receptor-type tyrosine kinase. c-MET is synthesized as a single-chain precursor (pr170) which undergoes post-translational glycosylation and proteolytic cleavage to give rise to the heterodimeric mature form.

Item	Value
Long Name	Hepatocyte Growth Factor Receptor
Entrez Gene IDs	4233 (Human); 17295 (Mouse)
Alternate Names	AUTS9, EC 2.7.10, EC 2.7.10.1, HGF R, HGF receptor, HGF/SF receptor, HGFR, MET, Met (c-Met), Proto-oncogene c-Met, RCCP2, SF receptor, Scatter factor receptor, Tyrosine-protein kinase Met, c-MET, cMET, hepatocyte growth factor receptor, met proto-oncogene (hepatocyte growth factor receptor), met proto-oncogene tyrosine kinase, oncogene MET

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human HGFR/c-MET Cartridge

Product Datasheets

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Protocols & Manuals

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