Simple Plex Human IFN-alpha 2 Cartridge

Name: Simple Plex Human IFN-alpha 2 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-002617

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-002617

Simple Plex Human IFN-alpha 2/IFNA2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

Customize Your Simple Plex Cartridge

Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.

Build My Cartridge

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.39 pg/mL
Assay Range:	3.64 - 13,870 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human IFN-alpha 2 Cartridge Summary:	Simple Plex assay for the detection of human IFN-alpha 2 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human IFN-alpha 2 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human IFN-alpha 2 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human IFN-alpha 2
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	46	45
Mean (pg/mL)	85	3742	81.7	3801
Standard Deviation	4.78	149	8.35	249
CV%	5.6	4	10.2	6.5

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	93	84-97
Heparin Plasma (n=4)	93	88-102
Serum (n=4)	96	88-100

Linearity

Samples containing and/or spiked with high concentrations of human IFN-alpha 2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human IFN-alpha 2 Cartridge

	Human IFN-alpha 2 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-alpha 2/IFNA2

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Item	Value
Long Name	Interferon alpha 2
Entrez Gene IDs	3440 (Human); 15965 (Mouse); 298213 (Rat)
Alternate Names	IFN-alpha 2, IFN-alpha-2, IFN-alphaA, IFNA, IFNA2, IFNA2a, IFNA2b, IFNA2c, IFNAA, IFNalpha 2, INFA2, Ifa2, Interferon alpha-A, LeIF A, MGC125764, MGC125765, alpha-2a interferon, interferon alpha 2b, interferon alpha A, interferon alpha-2, interferon, alpha 2

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

Learn More

Product Documents for Simple Plex Human IFN-alpha 2 Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol