Simple Plex Human LAG-3 Cartridge

Name: Simple Plex Human LAG-3 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-001348

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001348

Product Datasheet / COA

Simple Plex Human LAG-3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

Customize Your Simple Plex Cartridge

Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.

Build My Cartridge

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	15 pg/mL
Assay Range:	39.6 - 151,050 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human LAG-3 Cartridge Summary:	Simple Plex assay for the detection of human Lymphocyte Activation Gene 3 (LAG-3) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human LAG-3 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human LAG-3 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human LAG-3.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	61	60
Mean (pg/mL)	936	43772	972	45960
Standard Deviation	47.9	3730	81.5	4953
CV%	5.1	8.5	8.4	10.8

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	102	101-102
EDTA Plasma (n=4)	105	97-110
Heparin Plasma (n=4)	102	92-114
Serum (n=4)	97	95-98

Linearity

Samples containing and/or spiked with high concentrations of human LAG-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human LAG-3 Cartridge

	Human LAG-3 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LAG-3

LAG-3 (Lymphocyte activation gene-3; CD223) is a transmembrane protein that binds to MHC class II molecules and negatively regulates T cell receptor signaling. It is expressed on activated T cells, NK cells, and plasmacytoid dendritic cells (pDC). LAG-3 limits the expansion of activated T cells and pDC in response to select stimuli. Proteolytic shedding of LAG-3 enables normal T cell activation by removing the negative regulation. Binding of a homodimerized soluble LAG-3/Ig fusion protein to MHC class II molecules induces maturation of immature DC as well as secretion of pro-inflammatory cytokines by cytotoxic CD8+ T cells and NK cells.

Item	Value
Long Name	Lymphocyte-activation Gene 3
Entrez Gene IDs	3902 (Human); 16768 (Mouse); 297596 (Rat); 102122272 (Cynomolgus Monkey)
Alternate Names	CD223, CD223 antigen, LAG-3, LAG3, Secreted lymphocyte activation gene 3 protein, lymphocyte activating 3, lymphocyte activation gene 3 protein, lymphocyte-activation gene 3, sLAG-3

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

Learn More

Product Documents for Simple Plex Human LAG-3 Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol