Simple Plex Human Mesothelin Cartridge

Name: Simple Plex Human Mesothelin Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000511

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000511

Simple Plex Human Mesothelin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (25 uL)
Sensitivity:	2.29 pg/mL
Assay Range:	13.1 - 50,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Simple Plex Human Mesothelin Cartridge Summary:	Simple Plex assay for the detection of human Mesothelin in cell culture supernates (CCS), serum, plasma (EDTA/Heparin), and urine.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Mesothelin Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Mesothelin Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Mesothelin.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	22	19
Mean (pg/mL)	299	14366	296	14297
Standard Deviation	10	615	14.5	792
CV%	3.3	4.3	4.9	5.5

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	105	104-105

Linearity

Samples containing and/or spiked with high concentrations of human Mesothelin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Mesothelin Cartridge

	Human Mesothelin Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Mesothelin

Mesothelin, also known as CAK1 and ERC, is derived from a precursor that also includes Megakaryocyte Potentiating Factor (MPF). Following cleavage of the precursor, Mesothelin remains attached to the cell surface via a GPI linkage. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is upregulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression.

Item	Value
Entrez Gene IDs	10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)
Alternate Names	CAK1, CAK1 antigen, MPF, MPFSMRP, MSLN, Mesothelin, Pre-pro-megakaryocyte-potentiating factor, SMR, megakaryocyte potentiating factor, soluble MPF mesothelin related protein

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Mesothelin Cartridge

Product Datasheets

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COA

Protocols & Manuals

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