Simple Plex Human MICA Cartridge

Name: Simple Plex Human MICA Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000197

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000197

Product Datasheet / COA

Simple Plex Human MICA assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.31 pg/mL
Assay Range:	5.11 - 7,800 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human MICA Cartridge Summary:	Simple Plex assay for the detection of human MHC class I Chain-related gene A (MICA) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human MICA Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human MICA Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human MICA.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	20	18
Mean (pg/mL)	105	4434	103	5230
Standard Deviation	4.59	320	8.68	533
CV%	4.4	7.2	8.4	10.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=5)	105	101-109
EDTA Plasma (n=4)	91	73-102
Heparin Plasma (n=4)	96	87-102
Serum (n=4)	99	77-111

Linearity

Samples containing and/or spiked with high concentrations of human MICA were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human MICA Cartridge

	Human MICA Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MICA

MHC class I chain-related genes MICA and MICB are transmembrane glycoproteins that function as ligands for human NKG2D. The two proteins are highly related, sharing 85% amino acid identity, but are also polymorphic. Recognition of MICA or MICB by NKG2D results in the activation of NK cell cytolytic activity and/or cytokine production. MICA/B are minimally expressed on normal cells, but are frequently expressed on or shed from epithelial tumors and can be induced by bacterial and viral infections. MICA and MICB recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

Item	Value
Long Name	MHC Class I-related Protein A
Entrez Gene IDs	100507436 (Human)
Alternate Names	FLJ60820, MGC111087, MICA, PERB11.1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human MICA Cartridge

Product Datasheets

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COA

Protocols & Manuals

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