Simple Plex Human Nectin-2 Cartridge

Name: Simple Plex Human Nectin-2 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-006536

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-006536

Simple Plex Human Nectin-2/CD112 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	0.51 pg/mL
Assay Range:	6.55 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Nectin-2 Cartridge Summary:	Simple Plex assay for the detection of human Nectin-2 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Technical Data
Citations

Product Specifications for Simple Plex Human Nectin-2 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Specificity	Natural and recombinant human Nectin-2.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	38	38
Mean (pg/mL)	29.7	1618	29.2	1417
Standard Deviation	2.47	131.9	2.79	105
CV%	8.3	8.2	9.6	7.4

Linearity

Samples containing and/or spiked with high concentrations of human Nectin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human Nectin-2 Cartridge

	Human Nectin-2 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Nectin-2/CD112

Nectins are type I transmembrane glycoproteins that are calcium-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs). Homology has led to their designation as poliovirus receptor-related (PRR) proteins, but nectins do not bind poliovirus. The extracellular domains of the nectin family form cis-homodimers (same cell), followed by homophilic or heterophilic trans-dimers (across cells) that organize intercellular junctions. Nectin-1, also known as CD111, PRR1 and herpes virus entry mediator C (HVEC) has a secreted splice variant. Nectin-1, Nectin-2 (CD112, PRR2, HVEB) and Nectin-3 (PRR3) are concentrated in adherens junctions and exist on neurons, endothelial cells, epithelial cells and fibroblasts. Nectin-4 is mainly expressed in the placenta; a soluble form is generated via the action of TACE/ADAM17.

Item	Value
Long Name	Poliovirus Receptor Related 2
Entrez Gene IDs	5819 (Human); 19294 (Mouse); 102137456 (Cynomolgus Monkey)
Alternate Names	CD112, CD112 antigen, HVEB, HVEBpoliovirus receptor-like 2, Herpes virus entry mediator B, Herpesvirus entry mediator B, MPH, Nectin-2, Nectin2, PRR2, PRR2nectin-2, PVRL2, PVRR2poliovirus receptor related 2, herpesvirus entry protein B, nectin 2, poliovirus receptor-related 2 (herpesvirus entry mediator B), poliovirus receptor-related protein 2

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Nectin-2 Cartridge

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Protocols & Manuals

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