Simple Plex Human Osteopontin/OPN Cartridge

Name: Simple Plex Human Osteopontin/OPN Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000232

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000232

COA

Simple Plex Cartridge Kit for quantitation of OPN. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system.

Discontinued Product

SPCKB-PS-000232 has been discontinued. View all Osteopontin/OPN products.

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL), Urine (500 nL), Cerebrospinal Fluid (500 nL)
Sensitivity:	87.6 pg/mL
Assay Range:	87.6 - 21,390 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)
Simple Plex Human Osteopontin/OPN Cartridge Summary:	Simple Plex assay for the detection of human OPN in serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Osteopontin/OPN Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Osteopontin/OPN Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human OPN.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	16
Mean (pg/mL)	156	9737	161	8422
Standard Deviation	15.6	853	16.4	885
CV%	10	8.8	10.2	10.5

Linearity

Samples containing and/or spiked with high concentrations of human OPN were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Osteopontin/OPN Cartridge

	Human Osteopontin Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Osteopontin/OPN

Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell ad¬hesion, and migration. Its expression is upregulat¬ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.

Item	Value
Long Name	Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs	6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Alternate Names	BNSP, Bone sialoprotein 1, Eta-1, MGC110940, Nephropontin, OPN, Osteopontin, SPP-1, SPP1/CALPHA1 fusion, Spp1, Urinary stone protein, secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1), secreted phosphoprotein-1 (osteopontin, bone sialoprotein), uropontin

Compatible Instruments

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