Simple Plex Human PlGF Cartridge

Name: Simple Plex Human PlGF Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000261

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000261

Product Datasheet / COA

Simple Plex Human PIGF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.988 pg/mL
Assay Range:	3.6 - 5,490 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human PlGF Cartridge Summary:	Simple Plex assay for the detection of human PIGF in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human PlGF Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human PlGF Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human PIGF.
Cross-reactivity	Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	1
n	16	16	6	7
Mean (pg/mL)	34.9	1479	1614	31.8
Standard Deviation	1.56	73.6	178	1.89
CV%	4.5	5	11	6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=3)	91	87-93
Heparin Plasma (n=3)	86	78-91
Serum (n=3)	83	73-90

Linearity

Samples containing and/or spiked with high concentrations of human PIGF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human PlGF Cartridge

	Human PlGF Simple Plex Assay

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PlGF

PlGF (placenta growth factor) is a homodimeric growth factor that competes with VEGF for binding to VEGF R1/Flt-1. It therefore increases the availability of VEGF to bind to VEGF R2/KDR/Flk-1 and trigger angiogenesis. It can form heterodimers with some forms of VEGF and decrease the angiogenic effect of VEGF on VEGF R2. Circulating PlGF levels increase during pregnancy, reaching a peak in mid-gestation; this increase is attenuated in preeclampsia. PlGF induces monocyte activation and migration as well as production of inflammatory cytokines and VEGF. These activities facilitate wound, bone fracture, and cardiac repair, but also contribute to inflammation in active sickle cell disease and atherosclerosis. PlGF can also inhibit TIMP3 expression in the spleen, leading to immune triggering of hypertension.

Item	Value
Long Name	Placenta Growth Factor
Entrez Gene IDs	5228 (Human); 18654 (Mouse)
Alternate Names	D12S1900, PGF, PGFL, PLGFplacental growth factor-like, PlGF, PlGF-2, SHGC-10760, placenta growth factor, placental growth factor, placental growth factor, vascular endothelial growth factor-related protein

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human PlGF Cartridge

Product Datasheets

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COA

Protocols & Manuals

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