Simple Plex Human Reg3A Cartridge

Name: Simple Plex Human Reg3A Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000524

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000524

Product Datasheet / COA

Simple Plex Human Reg3A assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity:	1.93 pg/mL
Assay Range:	9.83 - 15,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Reg3A Cartridge Summary:	Simple Plex assay for the detection of human Reg3A in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Reg3A Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Reg3A Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Reg3A.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	24	22
Mean (pg/mL)	91	4305	82.1	4011
Standard Deviation	3.36	416	6.38	405
CV%	3.7	9.7	7.8	10.1

Linearity

Samples containing and/or spiked with high concentrations of human Reg3A were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Reg3A Cartridge

	Human Reg3A Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Reg3A

The Reg family constitutes a subset of the calcium-dependent C-type lectin superfamily. These small, secreted proteins have been implicated in a range of physiological processes including acting as acute phase reactants, lectins, survival/growth factors for insulin-producing pancreatic beta-cells, neural cells, and epithelial cells of the digestive system. Studies also indicate a role for Reg family members in tumor formation and indicate their potential for use as biomarkers of carcinogenesis.

Reg3A also named lithostathine 3 and pancreatitis-associated protein 2, belongs to the Regenerating gene family of C-type lectins. Reg3 is overexpressed during the acute phase of pancreatitis.

Item	Value
Long Name	Regenerating Islet-derived IIIa
Entrez Gene IDs	5068 (Human); 19694 (Mouse); 171162 (Rat)
Alternate Names	HIPpancreatitis-associated protein, Human proislet peptide, Lithostathine 3, PAP homologous protein, PAP1FLJ18565, PAP2, PAPPAP-H, PBCGF, Pancreatitis-associated protein 1, REG-3-alpha, REG-III, REG3, Reg III-alpha, Reg3A, Regenerating islet-derived protein III-alpha, hepatocarcinoma-intestine-pancreas, pancreatic beta cell growth factor, proliferation-inducing protein 34, proliferation-inducing protein 42, regenerating islet-derived 3 alpha, regenerating islet-derived protein 3-alpha

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Reg3A Cartridge

Product Datasheets

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COA

Protocols & Manuals

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