Simple Plex Human Resistin Cartridge

Name: Simple Plex Human Resistin Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-001017

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001017

Simple Plex Human Resistin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

Customize Your Simple Plex Cartridge

Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.

Build My Cartridge

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity:	0.35 pg/mL
Assay Range:	6.59 - 25,150 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Resistin Cartridge Summary:	Simple Plex assay for the detection of human Resistin in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Resistin Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Resistin Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Resistin.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	25	25
Mean (pg/mL)	137	7242	130	6913
Standard Deviation	13.5	460	14.4	644
CV%	9.9	6.4	11	9.3

Linearity

Samples containing and/or spiked with high concentrations of human Resistin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Resistin Cartridge

	Human Resistin Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Resistin

Resistin, also known ADSF and FIZZ3, is a secreted peptide hormone that circulates as either a noncovalent trimer, or a disulfide-linked homohexamer. Resistin is expressed by white adipocytes, splenocytes, astrocytes, and anterior pituitary epithelium. It blocks Insulin-stimulated uptake of glucose by adipocytes and promotes glucose release by hepatocytes. High circulating Insulin levels induce Resistin secretion which then antagonize Insulin action.

Item	Value
Entrez Gene IDs	56729 (Human); 57264 (Mouse)
Alternate Names	ADSF, ADSFMGC126609, Adipose tissue-specific secretory factor, C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, FIZZ3, FIZZ3MGC126603, HXCP1, RETN, RETN1, RSTNXCP1, Resistin, found in inflammatory zone 3

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

Learn More

Product Documents for Simple Plex Human Resistin Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol