Simple Plex Human TIM-3 Cartridge

Name: Simple Plex Human TIM-3 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-001049

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001049

Product Datasheet / COA

Simple Plex Human TIM-3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	0.33 pg/mL
Assay Range:	2.04 - 7,780 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human TIM-3 Cartridge Summary:	Simple Plex assay for the detection of human TIM-3 in serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human TIM-3 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human TIM-3 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human TIM-3.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	21	22
Mean (pg/mL)	34	1645	40.2	1964
Standard Deviation	2.76	125	4.4	196
CV%	8	8	11	10

Linearity

Samples containing and/or spiked with high concentrations of human TIM-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human TIM-3 Cartridge

	Human TIM-3 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIM-3

TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2 is an immunosuppressive protein that enhances tolerance and inhibits anti-tumor immunity. It is upregulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg). TIM-3 ligation by Galectin-9 attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. Dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens. TIM-3 also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA.

Item	Value
Long Name	T Cell Immunoglobulin Mucin-3
Entrez Gene IDs	84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)
Alternate Names	CD366, FLJ14428, HAVCR2, HAVcr-2, KIM-3, SPTCL, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, T-cell immunoglobulin and mucin domain-containing protein 3, TIM 3, TIM-3, TIM3, TIM3 T-cell membrane protein 3, TIMD-3, TIMD3, TIMD3KIM-3, hepatitis A virus cellular receptor 2, kidney injury molecule-3

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human TIM-3 Cartridge

Product Datasheets

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COA

Protocols & Manuals

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