Simple Plex Human TIMP-1 Cartridge

Name: Simple Plex Human TIMP-1 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-001442

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001442

Simple Plex Human TIMP-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL), Cerebrospinal Fluid (10 uL)
Sensitivity:	0.34 pg/mL
Assay Range:	1.05 - 10,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)
Simple Plex Human TIMP-1 Cartridge Summary:	Simple Plex assay for the detection of human Tissue Inhibitor of Metalloproteinases 1 (TIMP-1) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human TIMP-1 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human TIMP-1 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human TIMP-1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	32	33
Mean (pg/mL)	57.8	2915	54.5	2833
Standard Deviation	4.86	152	5.97	259
CV%	8.4	5.2	10.9	9.1

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	112	112-113

Linearity

Samples containing and/or spiked with high concentrations of human TIMP-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human TIMP-1 Cartridge

	Human TIMP-1 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIMP-1

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Item	Value
Long Name	Tissue Inhibitors of Metalloproteinases 1
Entrez Gene IDs	7076 (Human); 21857 (Mouse); 116510 (Rat)
Alternate Names	CLGI, Collagenase inhibitor, EPATIMP-1, EPO, Erythroid-potentiating activity, FLJ90373, Fibroblast collagenase inhibitor, HCI, TIMP metallopeptidase inhibitor 1, TIMP-1, TIMP1, TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, Tissue inhibitor of metalloproteinases 1, collagenase inhibitor), erythroid potentiating activity, metalloproteinase inhibitor 1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human TIMP-1 Cartridge

Product Datasheets

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Protocols & Manuals

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