Simple Plex Human VEGF-C Cartridge

Name: Simple Plex Human VEGF-C Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000224

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000224

Simple Plex Human VEGF-C assay kit for use on Ella instrument. Contains cartridge, sample diluent SD10, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (25 uL), Platelet-poor EDTA Plasma (25 uL), Platelet-poor Heparin Plasma (25 uL)
Sensitivity:	6.9 pg/mL
Assay Range:	32.6 - 49,690 pg/mL (Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
Simple Plex Human VEGF-C Cartridge Summary:	Simple Plex assay for the detection of human VEGF-C in serum and platelet-poor plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human VEGF-C Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human VEGF-C Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human VEGF-C.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	19	19
Mean (pg/mL)	149	7683	151	7918
Standard Deviation	13.7	531	13.4	727
CV%	9.2	6.9	8.8	9.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Platelet-poor EDTA Plasma (n=4)	111	92-122
Platelet-poor Heparin Plasma (n=4)	111	90-123

Linearity

Samples containing and/or spiked with high concentrations of human VEGF-C were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human VEGF-C Cartridge

	Human VEGF-C Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF-C

Vascular endothelial growth factor C (VEGF-C) is a homodimeric ligand of VEGF R3/Flt-4 and is synthesized with N- and C-terminal propeptides. Fully processed VEGF-C containing only the VEGF homology domain can additionally bind and activate VEGF R2/KDR/Flk-1. VEGF-C interactions with VEGF R3 are critical for lymphangiogenesis. Both the ligand and receptor are usually co-expressed at sites of lymphatic vessel sprouting, in the embryo, and in various pathological conditions. Over-expression of VEGF-C in tumor cells induces tumoral lymphatic hyperplasia, neoangiogenesis, and vessel sprouting, resulting in enhanced lymph flow and metastasis to regional lymph nodes.

Item	Value
Long Name	Vascular Endothelial Growth Factor C
Entrez Gene IDs	7424 (Human); 22341 (Mouse)
Alternate Names	Flt4 ligand, Flt4-L, VEGF-C, VEGFC, VRPFLT4 ligand DHM, Vascular endothelial growth factor-related protein, vascular endothelial growth factor C

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human VEGF-C Cartridge

Product Datasheets

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COA

Protocols & Manuals

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