Simple Plex Human VEGFR2/KDR/Flk-1 Cartridge

Name: Simple Plex Human VEGFR2/KDR/Flk-1 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000383

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000383

Simple Plex Human VEGFR2/KDR/Flk-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	14.9 pg/mL
Assay Range:	52.3 - 31,900 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human VEGFR2/KDR/Flk-1 Cartridge Summary:	Simple Plex assay for the detection of human VEGF R2 in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human VEGFR2/KDR/Flk-1 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human VEGFR2/KDR/Flk-1 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human VEGF R2.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	7	8
Mean (pg/mL)	222	10677	193	9248
Standard Deviation	12.6	529	24.3	944
CV%	5.6	5	12.6	10.2

Linearity

Samples containing and/or spiked with high concentrations of human VEGF R2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human VEGFR2/KDR/Flk-1 Cartridge

	Human VEGFR2/KDR/Flk-1 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGFR2/KDR/Flk-1

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Item	Value
Long Name	Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs	3791 (Human); 16542 (Mouse)
Alternate Names	CD309, CD309 antigen, EC 2.7.10, EC 2.7.10.1, FLK1tyrosine kinase growth factor receptor, Fetal liver kinase 1, Flk-1, Flk1, KDR, KRD1, Kinase insert domain receptor, Ly73, Protein-tyrosine kinase receptor flk-1, VEGF R2, VEGFR, VEGFR-2, VEGFR2, fetal liver kinase-1, kinase insert domain receptor (a type III receptor tyrosine kinase), soluble VEGFR2, vascular endothelial growth factor receptor 2

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human VEGFR2/KDR/Flk-1 Cartridge

Product Datasheets

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Protocols & Manuals

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