Simple Plex Mouse alpha-Fetoprotein/AFP Cartridge

Name: Simple Plex Mouse alpha-Fetoprotein/AFP Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-009926

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-009926

Product Datasheet / COA

Simple Plex Mouse AFP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

Customize Your Simple Plex Cartridge

Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.

Build My Cartridge

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	8.91 pg/mL
Assay Range:	74 - 112,883 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Mouse alpha-Fetoprotein/AFP Cartridge Summary:	Simple Plex assay for the detection of mouse alpha -Fetoprotein/AFP in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse alpha-Fetoprotein/AFP Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse alpha-Fetoprotein/AFP Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Specificity	Natural and recombinant mouse AFP.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	40	44
Mean (pg/mL)	732	39604	835	41220
Standard Deviation	42.3	2036	83.6	4015
CV%	5.8	5.1	10	9.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	92	90-94

Linearity

Samples containing and/or spiked with high concentrations of mouse AFP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Mouse alpha-Fetoprotein/AFP Cartridge

	Mouse alpha-Fetoprotein/AFP Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: alpha-Fetoprotein/AFP

alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. It is one of the earliest markers of the hepatocyte lineage. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.

Item	Value
Entrez Gene IDs	174 (Human); 11576 (Mouse); 24177 (Rat)
Alternate Names	AFP, Alpha-1-fetoprotein, DSCAM2, FETA, HP, HPAFP, alpha-Fetoprotein, alpha-fetoglobulin

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

Learn More

Product Documents for Simple Plex Mouse alpha-Fetoprotein/AFP Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol