Simple Plex Mouse Erythropoietin/EPO Cartridge

Name: Simple Plex Mouse Erythropoietin/EPO Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-009096

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-009096

Product Datasheet / COA

Simple Plex Mouse EPO assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	1.03 pg/mL
Assay Range:	7.86 - 12,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Mouse Erythropoietin/EPO Cartridge Summary:	Simple Plex assay for the detection of mouse Erythropoietin (Epo) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse Erythropoietin/EPO Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse Erythropoietin/EPO Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Specificity	Natural and recombinant mouse Epo.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	75	75
Mean (pg/mL)	77	3917	91.6	4348
Standard Deviation	2.13	96.2	10.8	363
CV%	2.8	2.5	11.8	8.3

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	103	103-103
EDTA Plasma (n=4)	94	89-100
Heparin Plasma (n=4)	83	77-90
Serum (n=4)	100	92-106

Linearity

Samples containing and/or spiked with high concentrations of mouse Epo were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Mouse Erythropoietin/EPO Cartridge

	Mouse Erythropoietin/EPO Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Erythropoietin/EPO

Erythropoietin (Epo) is a 30-kDa, heavily glycosylated protein produced primarily by the kidney. It is the principal factor that regulates erythropoiesis. The production of Epo by kidney cells is increased in response to hypoxia or anemia. The cDNAs for Epo have been cloned from many species. The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level.

The biological effects of Epo are mediated by the Erythropoietin receptor (Epo R). At the protein sequence level, the human Epo R is approximately 83% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding truncated soluble forms of Epo R have been found. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.

Item	Value
Entrez Gene IDs	2056 (Human); 13856 (Mouse); 24335 (Rat)
Alternate Names	ECYT5, EP, EPO, Erythropoietin, MGC138142, MVCD2, epoetin

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse Erythropoietin/EPO Cartridge

Product Datasheets

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COA

Protocols & Manuals

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