Simple Plex Mouse Granzyme B Cartridge

Name: Simple Plex Mouse Granzyme B Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-007963

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-007963

Simple Plex Mouse Granzyme B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Simple Plex Mouse Granzyme B Cartridge Summary:

Simple Plex assay for the detection of mouse Granzyme B in cell culture supernatant (CCS), serum, and EDTA plasma.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Technical Data
Citations

Product Specifications for Simple Plex Mouse Granzyme B Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Specificity	Natural and recombinant mouse Granzyme B.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Linearity

Samples containing and/or spiked with high concentrations of mouse Granzyme B were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Mouse Granzyme B Cartridge

	Mouse Granzyme B Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Granzyme B

Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.

Item	Value
Entrez Gene IDs	3002 (Human); 14939 (Mouse)
Alternate Names	C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, GRB, GZMB, Granzyme B, Granzyme-2, GranzymeB, GrzB, HLP, SECT

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse Granzyme B Cartridge

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Protocols & Manuals

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