Simple Plex Mouse IL-2 Cartridge

Name: Simple Plex Mouse IL-2 Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-003104

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-003104

Product Datasheet / COA

Simple Plex Mouse IL-2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (5 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	3 pg/mL
Assay Range:	3 - 1,827 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Mouse IL-2 Cartridge Summary:	Simple Plex assay for the detection of mouse IL-2 in cell culture supernatant (CCS), seru and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse IL-2 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse IL-2 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Specificity	Natural and recombinant mouse IL-2.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	16
Mean (pg/mL)	12.6	496	12.8	616
Standard Deviation	0.736	37	1.61	67.5
CV%	5.8	7.5	12.6	11

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	105	102-106
Heparin Plasma (n=4)	97	91-106
Serum (n=4)	100	93-108

Linearity

Samples containing and/or spiked with high concentrations of muose IL-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Mouse IL-2 Cartridge

	Mouse IL-2 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-2

IL-2 (Interleukin 2) is a critical cytokine in T cell biology. It promotes T cell activation and expansion, the development, maintenance and function of regulatory T cells (Treg), and the differentiation of CD8+ T cells into terminal effector cells and memory cells. IL-2 signals through a receptor complex composed of CD25/IL-2 R alpha, IL-2 R beta, and the Common gamma Chain (gamma c). IL-2 R beta is also a component of the IL-15 receptor complex. Gamma c is also a signaling subunit in the receptors for IL-4, -7, -9, -15, and -21.

Item	Value
Long Name	Interleukin 2
Entrez Gene IDs	3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names	Aldesleukin, IL-2, IL-2lymphokine, IL2, Proleukin, T cell growth factor, T-cell growth factor, TCGF, interleukin 2, interleukin-2, involved in regulation of T-cell clonal expansion

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse IL-2 Cartridge

Product Datasheets

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COA

Protocols & Manuals

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