Simple Plex Mouse/Rat M-CSF Cartridge

Name: Simple Plex Mouse/Rat M-CSF Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-008920

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-008920

Simple Plex Mouse/Rat M-CSF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Mouse Serum (10 uL), Rat Serum (10 uL), Mouse EDTA Plasma (10 uL), Rat EDTA Plasma (10 uL), Mouse Heparin Plasma (10 uL), Rat Heparin Plasma (10 uL)
Sensitivity:	0.12 pg/mL
Assay Range:	0.25 - 950 pg/mL (Cell Culture Supernates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Mouse Heparin Plasma, Rat Heparin Plasma)
Simple Plex Mouse/Rat M-CSF Cartridge Summary:	Simple Plex assay for the detection of mouse and rat Macrophage Colony Stimulating Factor (M-CSF) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse/Rat M-CSF Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse/Rat M-CSF Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse, Rat
Specificity	Natural and recombinant mouse and rat M-CSF.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Mouse Heparin Plasma, Rat Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	47	46
Mean (pg/mL)	5.74	280	3.72	191
Standard Deviation	0.39	16.6	0.33	15.5
CV%	6.9	5.9	9	8.1

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	98	96-100

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat M-CSF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Mouse/Rat M-CSF Cartridge

	Mouse/Rat M-CSF Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lack cleavage and PG sites and produce an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Item	Value
Long Name	Macrophage Colony Stimulating Factor
Entrez Gene IDs	1435 (Human); 12977 (Mouse)
Alternate Names	CSF-1, CSF1, Lanimostim, M-CSF, MCSF, MCSFlanimostim, MGC31930, colony stimulating factor 1 (macrophage), macrophage colony stimulating factor, macrophage colony-stimulating factor 1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse/Rat M-CSF Cartridge

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Protocols & Manuals

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