Simple Plex Mouse/Rat SP-D Cartridge

Name: Simple Plex Mouse/Rat SP-D Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-008605

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-008605

Simple Plex Mouse/Rat SP-D assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Mouse Bronchoalveolar Lavage (10 uL), Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	1.8 pg/mL
Assay Range:	7.06 - 26,909 pg/mL (Bronchoalveolar Lavage Fluid, Mouse Bronchoalveolar Lavage, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Mouse/Rat SP-D Cartridge Summary:	Simple Plex assay for the detection of mouse and rat Surfactant Protein D (SP-D) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) and Bronchoalveolar Lavage Fluid (BALF). BALF are validated for mouse samples only.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse/Rat SP-D Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse/Rat SP-D Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse, Rat
Specificity	Natural and recombinant mouse and rat SP-D.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Bronchoalveolar Lavage Fluid, Mouse Bronchoalveolar Lavage, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	44	42
Mean (pg/mL)	187	9028	201	9644
Standard Deviation	13.8	826	25.3	1132
CV%	7.4	9.2	12.6	11.7

Recovery

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	92	82-102

Linearity

Samples containing and/or spiked with high concentrations of SP-D were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Mouse/Rat SP-D Cartridge

	Mouse/Rat SP-D Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SP-D

Surfactant Protein D (SP-D) is a collectin family protein consisting of a collagen-like sequence followed by a C-type lectin domain. It is synthesized primarily in the lung by alveolar type II cells and unciliated bronchial epithelial cells. In addition, SP-D is produced by epithelial cells of the gastrointestinal tract and exocrine glands. SP-D binds to patterns of neutral sugars on micro-organisms to mediate direct opsonization and phagocytosis and is an important component of the innate immune system.

Item	Value
Long Name	Surfactant Pulmonary Associated Protein D
Entrez Gene IDs	6441 (Human); 20390 (Mouse)
Alternate Names	COLEC7collectin-7, Collectin 7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, SFTP4, SFTP4pulmonary surfactant-associated protein D, SFTPD, SP-D, SP-Dpulmonary surfactant apoprotein, SPD, surfactant protein D, surfactant, pulmonary-associated protein D, surfactant-associated protein, pulmonary 4

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse/Rat SP-D Cartridge

Product Datasheets

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Protocols & Manuals

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