Simple Plex Neuro Panel 1

Name: Simple Plex Neuro Panel 1
Brand: ProteinSimple
SKU: SPCKC-PS-009830

ProteinSimple, part of Bio-Techne | Catalog # SPCKC-PS-009830

COA

Simple Plex 32x2 panel containing GFAP 2nd Gen and NF-L with sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 3-5 days. Sample type indicated with PS (Plasma/Serum).

Key Product Details

Features:

The Simple Plex Human Multianalyte Assay is for the detection of human GFAP 2nd Gen and NF-L in serum and plasma (EDTA/Heparin).

Sample Type & Volume Required Per Well	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)
Sensitivity	GFAP 2nd Gen 0.64 pg/mL; NF-L 1.09 pg/mL
Assay Range	GFAP 2nd Gen 2.52 - 9,600 pg/mL; NF-L 2.7 - 10,290 pg/mL

Technical Data
Citations

Summary for Simple Plex Neuro Panel 1

Product Specifications

Reactivity	Human
Specificity	Natural and recombinant human GFAP 2nd Gen and NF-L.
Cross-reactivity
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

		Intra-Assay Precision		Inter-Assay Precision
	Sample	1	2	1	2
GFAP 2nd Gen	n	16	16	62	59
	Mean (pg/mL)	75.1	3705	76.3	3529
	Standard Deviation	3.54	218	6.08	257
	CV (%)	4.7	5.9	8	7.3
NF-L	n	16	16	48	48
	Mean (pg/mL)	76.6	3309	80.5	3476
	Standard Deviation	3.64	154	8.35	267
	CV (%)	4.8	4.7	10.4	7.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

	Sample Type	Average % Recovery	Range %
GFAP 2nd Gen	EDTA Plasma (n=4)	94	90-98
	Heparin Plasma (n=4)	95	90-102
	Serum (n=4)	91	86-100
	Cell Culture Media (n=2)	110	110-111
NF-L	EDTA Plasma (n=4)	93	86-99
	Heparin Plasma (n=4)	101	93-106
	Serum (n=4)	108	95-115
	Cell Culture Supernates (n=5)	12	110-115

Product Specifications for Simple Plex Neuro Panel 1

Species

Human

Preparation & Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Simple Plex Neuro Panel 1

ProteinSimple, part of Bio-Techne | Catalog # SPCKC-PS-009830

Key Product Details

Summary for Simple Plex Neuro Panel 1

Product Specifications

Precision

Recovery

Product Specifications for Simple Plex Neuro Panel 1

Preparation & Storage

Product Documents for Simple Plex Neuro Panel 1

COA

Certificate of Analysis

CONTACT INFORMATION

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