Simple Plex Protein A Cartridge

Name: Simple Plex Protein A Cartridge
Brand: ProteinSimple
SKU: SPCKB-OT-009204

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-OT-009204

Simple Plex Protein A assay kit contains cartridge, sample diluent SD62, and wash buffer. Part # starting with ST01 ships in 2-3 days.

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Bioprocess Samples (25 uL)
Sensitivity:	4.34 pg/mL
Assay Range:	11.5 - 17,500 pg/mL (Bioprocess Samples)
Simple Plex Protein A Cartridge Summary:	Simple Plex assay for the detection of natural and engineered variant Protein A in Bioprocess samples.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Protein A Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Protein A Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Bioprocess
Specificity	Natural and engineered variant Protein A in Bioprocess samples.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Bioprocess Samples

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	29	27
Mean (pg/mL)	216	8267	167	7065
Standard Deviation	4.6	234	19.8	642
CV%	2.1	2.8	11.8	9.1

Recovery

Recovery at four different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Bioprocess Samples (n=6)	97	90-103

Linearity

Samples containing and/or spiked with high concentrations Protein A were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Protein A Cartridge

	Protein A Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Protein A

Protein A is a Staphylococcus aureus cell wall constituent that is characterized by its binding affinity to the Fc portion of some immunoglobulins, especially those of the IgG class. Because of this property, it is often utilized for microscopic visualization of molecules and to purify antisera. Protein A has also been used for the treatment of proteinuria in nephrotic syndrome, and severe autoimmune diseases such as rheumatoid arthritis, coeliac disease, and systemic lupus erythematosis.

Item	Value
Entrez Gene IDs	3919448 (S. aureus)
Alternate Names	Immunoglobulin G binding protein A, PROA, Protein A, Protein-A, SPA, Staphylococcal protein A

Compatible Instruments

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Product Documents for Simple Plex Protein A Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol