Lysis Kit - Bicine/CHAPS Buffer for Charge Assays
ProteinSimple, part of Bio-Techne | Catalog # CBS403
Compatible with Peggy Sue or NanoPro 1000 systems
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Key Product Details
- Bicine/CHAPS Lysis Buffer contains 20 mM Bicine (pH 7.5), 0.6% CHAPS and 1X DMSO and aqueous inhibitor mixes.
- Aqueous Protease Inhibitor contains 40mM NaF, 12 mM β-glycerophosphate, 1 mM Sodium Orthovanadate, 0.5mM EDTA, 2.5 mM EGTA
- DMSO Inhibitor Mix contains 1mM AEBSF, 5µg/ml Aprotinin, 50 µM Bestatin, 5µg/ml E-64 Protease Inhibitor, 10 µg/ml Leupeptin, 7µg/mL Pepstatin A, Phosphatase inhibitor cocktail (with effective concentrations of Cantharidin, Bromotetramisole and Microcystin LR)
- Cell Wash Buffer contains 20 mM Bicine (pH 7.5), 250 mM Sucrose